Nucleotide precursor in riboflavin biosynthesis.

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Biosynthesis of Riboflavin.

The biosynthesis of riboflavin requires 1 equivalent of GTP and 2 equivalents of ribulose phosphate. The first committed reactions of the convergent pathway are catalyzed by GTP hydrolase II and 3,4-dihydroxy-2-butanone 4-phosphate synthase. The initial reaction steps afford 5-amino-6-ribitylaminopyrimidine 5'-phosphate, which needs to be dephosphorylated by a hitherto elusive hydrolase. The de...

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Riboflavin as precursor in the biosynthesis of the 5,6-Dimethylbenzimidazole-moiety of vitamin B(12).

The 5,6-dimethylbenzimidazole formed from riboflavin in cobalt-containing cells of Propionibacterium shermanii can only be isolated as a component of vitamin Blz. No free 5,6-dimethylbenzimidazole is detectable in these bacteria. In order to determine, if nevertheless the vitamin Bn biosynthesis proceeds via free 5,6-dimethylbenzimidazole, cells of P. shermanii were incubated with [5-‘“Nlribofl...

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Biosynthesis of riboflavin. Structure of the purine precursor and origin of the ribityl side chain.

We studied the incorporation of 14C-labeled guanosine into riboflavin under conditions precluding the metabolic conversion of guanosine compounds to free guanine. For this purpose we isolated a mutant BM 2 of Salmonella typhimurium deficient in the enzymes IMP dehydrogenase, purine nucleoside phosphorylase, and purine nucleotide pyrophosphorylase. The mutant incorporated [ribose-14C]guanosine i...

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Transfer RNA biosynthesis: the nucleotide sequence of a precursor to serine and proline transfer RNAs.

The nucleotide sequence of a transfer RNA precursor molecule coded by bacteriophage T4 has been determined. The molecule is a single polynucleotide chain which contains two transfer RNA species that are destined to recognize serine and proline. The 3' -CCA(OH) termini of both mature transfer RNA species are absent in the precursor molecule; these termini must therefore be added enzymatically at...

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Precursor directed biosynthesis of aureobasidins.

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ژورنال

عنوان ژورنال: Journal of Nutritional Science and Vitaminology

سال: 1976

ISSN: 0301-4800,1881-7742

DOI: 10.3177/jnsv.22.477